By contrast, thermal cyclers use electric heating and cooling components to drive the PCR reaction through each of its three phases: denaturation, annealing and extension. During denaturation ...

since the annealing temperature of the fluorescent dye-labeled M13(-21) primer is only 53°C, the last eight PCR cycles should be run with an annealing temperature of 53°C (the same result can be ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

This video illustrates the PCR process. It begins with denaturation, where the DNA template strands separate. Next, primers anneal to the target sequences during the annealing step. Finally, DNA ...

Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer ...