7| Cut a piece of nylon or nitrocellulose membrane and two sheets of thick blotting paper ∼1 mm larger than the gel in each dimension. Cut a corner from the membrane to match the gel (step 5).

3| Place a piece of thick blotting paper on a sheet of Plexiglas or a glass plate to form a support that is longer and wider than the gel. Drape the ends of the blotting paper over the edges of ...