We prepared squashed 10 and spread 14 spermatogenic and oogenic cells as described, using 2% formaldehyde containing 0.05% Triton X-100 and 0.02% SDS as the fixative in both cases. BrdU labeling ...

was placed subcutaneously to administer BrdU (1 mg/d) for 7 d. We determined serum ALT concentrations using a colorimetric diagnostic kit (Sigma). We transiently transfected 293T and 293TGP cells ...

Microglia are brain-resident macrophages playing pivotal roles in CNS development and homeostasis. Yet, the cellular and molecular basis governing microglia maintenance ...