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A75-3.1 and A75-2.1. Anti-human CD3 (clone UCHT-1) and CD4 (clone Q4120) were from Sigma and anti-human CD14 (clone RMO52) from Coulter Immunotech (Luton, UK). Synthesis of biotin-agarose.
Freshly isolated mononuclear cells (MNCs) were stained with CD45- CD3-, CD14-and CD19-, CD56-, CD16-, CD57, CD62L-, CD27- and CD45RA-antibodies and analysed with flowcytometry. For more detailed ...
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