A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

Additionally, the authors create whole-genome Cas12a knockout libraries, and demonstrate their utility across multiple in vitro and in vivo screens. CRISPR-based detection has long been challenged ...

(Image: Doudna Lab, UC Berkeley) In the CRISPR-Cas9 system, the guide RNA directs the Cas9 protein to the target DNA sequence, where it creates a double-strand break. This break can then be repaired ...

Both gene knockout and knock-in models are powerful ... regularly interspaced short palindromic repeats (CRISPR)/associated ...