With CRISPR-Cas9, the crRNA/tracrRNA sequence or an artificial guide RNA indicate where the DNA can be cut. It is relatively easy for scientists to produce different sequence variants from RNA ...

Single guide RNAs (sgRNAs) designed and built to guide Cas9 to bind and cut desired intronic regions in the EML4 and ALK gene targets, trigger the paracentric genomic rearrangement event upon co ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Continue reading about off-target effects in this story. Base and Prime Editing Offer Precision CRISPR-based systems that rely on traditional Cas nucleases like Cas9 cut through both strands of DNA ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.

Danielle is a Senior Editor at The Scientist. She has a background in neuroscience. Her science communication experience spans journalism, scientific publishing, and science education and outreach.

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...

The results appear online March 14 in the Proceedings of the National Academy of Sciences (PNAS). CRISPR-Cas9 burst onto the broader scientific scene in 2012 when a team led by Jennifer Doudna showed ...

What happens next can vary. The standard Cas9 protein cuts the DNA at the target. When the cut is repaired, mutations are introduced that usually disable a gene. This is by far the most common use ...

it’s also important to recognize the steps and precautions Atkin took in this experiment and to keep in mind that all CRISPR-Cas9 experiments should be done with safety precautions. Here on campus, ...