Danielle is a Senior Editor at The Scientist. She has a background in neuroscience. Her science communication experience spans journalism, scientific publishing, and science education and outreach.

For example, the commonly used Cas9 is about 1,400 amino acid residues long ... The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Therefore, when these bacteria are infected, the transcripts of these repeats guide a nuclease to the complementary DNA of the virus and destroy it. 3 Scientists adopted CRISPR-Cas9 as a gene ...

CRISPR-Cas9 is not the first method available to scientists for modifying DNA; it is by far, however, the easiest to use. With CRISPR-Cas9, the crRNA/tracrRNA sequence or an artificial guide RNA ...

However, recent research uncovers a novel mechanism in this complex that differs significantly from the well-known type II CRISPR systems like Cas9 ... with the HNH nuclease domain.

Despite its advantages, TALEN also faces some challenges. The construction of TALEN proteins can be time-consuming and labor-intensive compared to other gene editing tools like CRISPR-Cas9.

The editing event is based on a double strand break caused by a Cas9 nuclease, the specificity of which is defined by a guiding sequence of RNA that is complementary to desired site in the organism’s ...