He describes the challenge as follows: “You have to elute directly from the bait, and then you have hundreds of proteins that bind nonspecifically, and you need to analyze them all.” ...

8| Optional: Elute the GST fusion protein and any proteins bound to it by adding 50 μl of 20 mM reduced glutathione in 50 mM Tris-Cl (pH 8.0) to the beads. Centrifuge the tubes at maximum speed ...