The findings were published last week (February 12) in Science Advances. “[This paper] is another cautionary tale about the use of CRISPR-Cas9–based gene editing for [knock-in] purposes,” remarks Ed ...

CRISPR-Cas9, a gene editing technology known for its user-friendliness, can knock genes in or out. Knocking out a gene involves inserting CRISPR-Cas9 into a cell using a guide RNA that targets the ...

While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR ...