all bacteria nonetheless have just a single replication origin (Mackiewicz et al., 2004). Also, whereas bacterial chromosomes are circular, eukaryotic chromosomes are linear. During circular DNA ...

Cip/Kip proteins that inhibit cyclin-dependent kinase 2 (Cdk2) restrain the initiation of DNA replication ... depends on the presence of the Mcm2–7 origin proteins (Fig. 1, right).

Over 20 distinct proteins and protein complexes, comprising over 50 gene products, are minimally required to establish replisomes at eukaryotic replication origins. We have reconstituted this process ...