Each of the fragments shared a segment of an overlapping sequence with one or two other fragments. Figure 1: Schematic diagram of the PCR–mediated recombination strategy used in constructing HIV ...

One single fluorescent dye-labeled primer with the M13(-21) sequence can be used for all fragments. The method can even be multiplexed, if the various PCR products can be size-fractionated ...

"Fighting Fraud with DNA"1 claims that high-speed PCR is "too expensive now, but probably not in 10 or 20 years. There will have to be some breakthroughs in amplifying and reading faster ...." We have ...