Each of the fragments shared a segment of an overlapping sequence with one or two other fragments. Figure 1: Schematic diagram of the PCR–mediated recombination strategy used in constructing HIV ...

There will have to be some breakthroughs in amplifying and reading faster ...." We have already built PCR instruments that amplify DNA fragments of 100 to 2000 base pairs through 30 PCR cycles in 1.3 ...

We amplified multiple overlapping mtDNA fragments by PCR from the extracts, cloned the products and sequenced the insertions of multiple clones. When using primers that allowed both modern human ...