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Pipet the emulsion into the wells of a PCR plate as 10 aliquots of 50 μl. Pipet 50 μl of the aqueous phase (Step 3) into a well as a nonemulsified control. Overlay the emulsified and ...
PCR plates and microplates usually require some type of seal to prevent evaporation, which otherwise may alter sample concentrations and impair repeatability. Manual sealing and peeling of ...
Add, in order, one steel bead, 600 μl oil-emulsifier mix (Step 9) and 150 μl PCR mix to one well of a 96-well storage plate. Seal plate with adhesive film. Turn the plate upside down to make ...
The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. This allows all the ...
The Glo-Plate™ 2.0 delivers exceptionally uniform LED illumination for viability PCR (v-PCR) applications, offering ideal photoactivation for PMAxx™– or PMA-treated samples with minimal exposure of ...
For instance, labs using Thermo Scientific ™ KingFisher ™ instruments with deep well plates prior to next generation sequencing (NGS) library preparation or quantitative PCR (qPCR) can now ...
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