Polymerase chain reaction, or PCR, is a laboratory technique ... the temperature of the sample in automatic, programmed steps. Initially, the mixture is heated to denature, or separate, the ...

2| Remove 48 μl to serve as the no-template control reaction and add 2 μl of primer pair 1. 3| Add 5.5 μl of genomic DNA at 10 ng/μl to the master mix (Step 1) and mix thoroughly. 4 ...