RNA polymerases are sloppy. A retrovirus with an RNA genome, which is copied into DNA by a reverse transcriptase, is prone to mutations because the enzyme lacks the 3’ to 5’ proofreading capability of ...

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

As the coronavirus that causes the COVID-19 disease spreads across the world, the IAEA, in partnership with the Food and Agriculture Organization of the United Nations (FAO), is offering its support ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...

The four main classes, which most people are treated with, target one of three viral proteins which control HIV’s lifecycle: reverse transcriptase, integrase and protease. Historically, most people ...

2| Set up the following reverse transcription reaction and three controls: Incubate the reactions at 37 °C for 60 min. The success or failure of 5′ RACE is determined here at the beginning of ...

More information: Kaiyang Zheng et al, Diversity of reverse-transcriptase-containing viruses through global metagenomics, hLife (2024). DOI: 10.1016/j.hlife.2024.10.002 Provided by Tsinghua University ...

The COVID-19 outbreak in 2019 triggered measures to raise public awareness regarding pandemics and also led to fast-tracked ...

It is an ester-derived prodrug that is converted in vivo by serum and tissue esterases to tenofovir, an acyclic nucleoside phosphonate (nucleotide) that inhibits HIV reverse transcriptase.