and a few others unpack the facility's first next-generation sequencing machine, a 454 GS FLX, on loan from Roche Diagnostics for three months. They train for a few days, nebulize a colleague's ...

The Roche/454 GS FLX System is based on emulsion PCR [8] and pyrophosphate detection [9] techniques. A library of DNA templates is constructed by a highly efficient DNA amplification method known ...

DNA sequencing is a powerful approach for ... an overview of four commonly used NGS technologies from Roche Applied Science//454 Life Sciences, Illumina, Life Technologies and Helicos Biosciences.