1| Wash thirty 10-cm plates of the appropriate cells (a total of ∼6 × 10 7 cells) in phosphate-buffered saline. Scrape each ... (20 mM Tris (pH 8), 1 mM EDTA, 900 mM NaCl, 0.5% NP-40).

Wash the cell pellet with 10 ml of ice-cold 1× phosphate-buffered saline. Pellet cells at 300g ... MNase stop buffer consists of 100 mM EDTA and 10 mM EGTA (pH 7.5). 8| Extract samples with ...