surface-exposed residues were more aggregation-resistant than their neutral counterparts. They made supercharged variants of GFP as well as glutathione S-transferase and streptavidin, and found ...

Reengineered streptavidin mutants bind biotin-4-fluorescein with extremely tight yet reversible binding affinity. The terrifically tight noncovalent interaction between biotin and streptavidin has ...