Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells 1,2 ... But as with other transfection methods, the optimal conditions for electroporation of untested ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...