Thus, small- to large-scale transient transfection of mammalian cells growing in suspension is needed. However, because of the very high cost of transfection reagents, adoption of this modern ...
1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...