Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

One to three hours before transfection, exchange the ES cell medium with fresh medium. Treat an appropriate number of tissue culture plates with gelatin solution, adding enough to cover the bottom ...