Transformation is a genetic exchange mechanism by which “naked” DNA is taken ... Using a P-200 pipetman, add 100 microliters of competent E. coli to the "-DNA" tube. Place the tube back on ice. 7.

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli ... When the transformation efficiency is critical (for example, when one plasmid ...