To complete our studies, we substituted emerald GFP (emGFP) for YFP. Using two-photon excitation, CFP can be excited at 800–825 nm with little direct excitation of emGFP (which has two-photon ...

Interestingly, the authors were also able to insert an entire protein, the calcium-sensitive calmodulin (CaM), into GFP or YFP, the yellow cousin of GFP. The putative structure of a GFP–CaM ...