The basic concept behind the extended depth of field is that a specially designed phase plate is inserted at the back aperture of the microscope objecive. The phase plate creates a uniquely shaped PSF ...

The SEM has allowed researchers to examine a much bigger variety of specimens. The scanning electron microscope has many advantages over traditional microscopes. The SEM has a large depth of field, ...

The microscope works best on something flat and has a very shallow depth of field, so anything poking in our out will probably be out of focus. The unit did, however, look substantial and had a ...