Poly(A) + mRNA is preferred as a template for ... Transfer the retentates containing cDNA to fresh 0.5-ml microcentrifuge tubes, and reduce the volumes to ∼10 μl in a rotary vacuum evaporator.

The reaction can then be run in a thermocycler. A poly (A) tail is first added to the RNA template, and then cDNA is synthesized using an adaptor primer. In addition to its simplicity, the single ...