Coamplification at lower denaturation temperature (COLD)-PCR is a PCR-based application that preferentially enriches the amplification of mutants and variants throughout the course of ...

PCR was developed in 1983 ... DNA or cDNA as a template during repeated cycles of heat denaturation, primer annealing and primer extension. With each cycle the amount of template DNA is doubled ...

One promising avenue is the adoption of PCR, encompassing Pre-Consumer or Post-Consumer Recycled materials. This approach involves repurposing recycled plastic sourced from end-users or utilising ...

The CNT coating between the layers is heated with microwaves to locally anneal. (Credit: Sweeney et al., Science Adv., 2017) Layer adhesion is one of the weak points with FDM 3D printing ...

The reverse transcription reaction was carried out under the following conditions: primer annealing at 25°C for 2 min, cDNA synthesis at 55°C for 30 min, and heat inactivation at 95°C for 1 min. The ...

AI constraint solver in Java to optimize the vehicle routing problem, employee rostering, task assignment, maintenance scheduling, conference scheduling and other planning problems.

A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

OpenJij : Framework for the Ising model and QUBO.

H9N2 viral RNA concentration was measured from fallopian tube and ovarian tissues in breeder goose infected with H9N2 AIV at 1, 3, 5, and 7 dpi using quantitative SYBR Green I real-time PCR (qRT-PCR) ...

This study provides a valuable new resource to investigate the molecular basis of the particular features characterizing the pipefish embryo. The authors found both unique and shared gene expression ...

PCR was done at 40 cycles of 20 s denaturation (at 94 °C), 20 s annealing (at 55 °C), and 20 s extension (at 72 °C). Antibodies were labeled by chloramine-T–catalyzed iodination and purified ...