来自MSN14 小时
CRISPR-Cas9 Off-Target Effects: Challenges and Solutions
The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...
The EMBO Journal4 天
A CRISPR-Cas9 screen reveals genetic determinants of the cellular response to decitabine
A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...
生物通1 年
CRISPR/Cas9基因编辑sgRNA转染优选
Xfect RNA转染试剂也用于高效转染单向导RNA(sgRNA)进行CRISPR ... Transfection Reagent 可将sgRNA有效转染至HT1080细胞 Panel A. 以CD81反义链5’端序列为模板，采用Guide-it sgRNA In Vitro Transcription Kit合成sgRNA。 Panel B. 采用Xfect RNA转染试剂转染50 ...
The Scientist11 个月
Dharmacon™ Edit-R™ Gene Engineering System
A plasmid encoding Cas9 nuclease, (2) a chemically-synthesized trans-activating RNA (tracRNA), and (3) a custom CRISPR RNA (crRNA) designed to target the site of interest. The Edit-R platform greatly ...
New Scientist5 年
What is CRISPR?
When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...
Helsinki23 天
CELL LINE SERVICES
FINGEEC provides validation of the customer designed or commercial gRNAs in HEK293FT cells prior to generating CRISPR/Cas9 knockout cell lines. We check the transfection efficacy of the constructs ...
CNN1 年
CRISPR Therapeutics AG
CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.