This valuable study presents a mouse gastruloid model that can be used to generate hematopoietic progenitors as well as leukemic cells. However, in its current form, the manuscript is inadequate ...

Until now, component analysis of culture medium has been carried out by periodic sampling; however, this method only allows for small samples to be collected and requires regular manual sampling, ...

Drosophila S2 cells were transfected using PEI (Polyethylenimine, 1 μg/μl) with final DNA concentration of 1 μg/ml and final PEI concentration of 3 μg/ml in total culture media. Drp1-HA, Mfn-HA, ...

The target proteins phosphoglycerate kinase 2 (PGK2), glycerol-3-phosphate dehydrogenase ... the HepG2 cells were treated or not treated with 0–200 µM esculetin (0.9% NaCl solution containing 1% EtOH) ...

Convincing evidence shows that the sphingosine-1-phosphate ... room temperature in a buffer consisting of 100 mM Tris, 8 mM CuSO 4, and 100 mM ascorbic acid in dH 2 O. The Alexa Fluor 647 Azide ...

1 Department of Medicine, Division of Gastroenterology & Hepatology, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK 2 Wellcome Trust PhD Programme for Clinicians, Cambridge Institute ...

Battling a chronic disease is like running a marathon where T cells, the immune system’s frontline defenders, are the runners. When the starter’s pistol fires, the runners are fast and strong.