RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA ...

Here we describe bisulfite sequencing assays and bioinformatic processing methods to identify cfDNA derived ... Briefly, 500 μg of resuspended VX Carrier RNA (Qiagen, 950280) was added to the ...

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The Bio-IT Centre at IBAB is organizing a three-day hands-on workshop on ”Transcriptome library preparation and RNA-seq analysis” on February 22-24, 2025 . This workshop will provide hands-on ...

Methods: We constructed nine DNA methylomes at different time points after allotransplantation and used bisulfite genomic sequencing PCR technology (BSP) to verify the methylation status in the ...

CRISPR epigenome editing successfully reactivates silenced genes in Prader-Willi Syndrome, highlighting potential for ...

How WBL’s proprietary nanopore-based approach eliminates PCR and bisulfite-induced biases, delivering accurate, biologically relevant insights. How WBL’s sequencing provides a clear ...

It does so by combining technologies such as DNA and RNA sequencing and proteomics – the study of the way proteins interact with one another – to create a molecular profile of a patient’s cancer.

他们通过 CRISPR-Cas9 基因编辑技术构建了番茄 SlNRPD1 基因的敲除突变体；利用 sRNA 测序（sRNA-seq）分析了突变体和野生型胚胎中小 RNA（sRNA）的表达谱变化；借助全基因组亚硫酸氢盐测序（whole-genome bisulfite sequencing）检测了 DNA 甲基化水平的改变；还使用 RNA 测序 ...