Frizzled (FZD) proteins are the principal receptors of the Wnt signaling pathway. However, whether Wnt ligands induce FZD endocytosis and degradation remains elusive. The transmembrane E3 ubiquitin ...

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(A and B) Image of Ki67-positive cells (Red: Ki67, Blue: DAPI) and their quantification at the crypt base of proximal jejunum (A) or colon (B) of NIC-treated and untreated mice (A:3–4 mice per group, ...

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Cells were permeabilized in 0.2% Triton-X-100 in PBS (Sigma-Aldrich, cat. no. T8787-50 mL) for 2–5 minutes. Next 2×PBS washes were performed, after which DAPI was added at 1 μg/mL for 90 min at room ...