Desmin mutations impact the autophagy flux in C2C12 cell in …
In the present study, we focused on alteration of autophagy flux in myoblasts expressing various Des mutations. We applied Western blotting, immunocytochemistry, RNA sequencing, and shRNA approach to demonstrate that Des S12F, Des A357P, …
Anti-Desmin Antibodies | Invitrogen - Thermo Fisher Scientific
Antibodies that detect Desmin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. These antibodies target Desmin in Human, Rat, Mouse, Chicken and Hamster samples. Our Desmin monoclonal, polyclonal and recombinant monoclonal...
Use of desmin immunohistochemistry to distinguish between mesothelial ...
Objective: To evaluate the utility of immunohistochemical stains for desmin in discriminating mesothelial cells from adenocarcinoma in serous fluid cell block preparations. Study design: Cell block preparations from 22 cases (representing 18 patients) that were positive for carcinoma and 5 cases that were negative for malignancy were ...
Anti-Desmin Antibodies | Invitrogen - 赛默飞世尔科技公司
Antibodies that detect Desmin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. These antibodies target Desmin in Human, Rat, Mouse, Chicken and Hamster samples. Our Desmin monoclonal, polyclonal and recombinant monoclonal...
Immunocytochemical detection of desmin in fat-storing cells
We obtained desmin antibodies from New Zealand white rabbits which were immunized with desmin following its extraction from chicken gizzard smooth muscle. By immunocytochemical methods, the antibody stained the Z-line of rat skeletal myofibrils and produced only one band with chicken gizzard desmin by immunoblotting.
Monoclonal antibodies to desmin: evidence for stage-dependent …
Monoclonal antibodies reactive with desmin (D3 and D76) have been generated and their specificities validated by immunoblots, RIAs, and immunocytochemistry. No cross-reaction with other IFPs has been observed. The McAbs recognized different epitopes but both reside in the amino-terminal rod domain of desmin.
Desmin Antibody (D93F5) | Cell Signaling Technology
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy terminal residues of human desmin protein. The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.
Antioxidant Treatment and Induction of Autophagy Cooperate …
2015年9月2日 · The desmin gene (DES) encodes the 53-kDa muscle-specific intermediate filament (IF) desmin. Desmin forms a sarcoplasmic network that encircles Z disks to connect them and binds them to the sarcoplasmic membrane and the nuclear lamina [12–14].
In the present study, we focused on alteration of autophagy flux in myoblasts expressing various Des mutations. We applied Western blotting, immunocytochemistry, RNA sequencing, and shRNA approach to demonstrate that DesS12F, DesA357P, …
Desmin Monoclonal Antibody (DE-U-10) (14-9747-82)
Applications Tested: This DE-U-10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL.