Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB), [2] albutest [3] is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid. [4]

Bromophenol Blue | C19H10Br4O5S | CID 8272 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels ...

Bromophenol blue is a pH indicator, yellow below pH 4 and blue above that pH. Therefore, if the SDS sample buffer turns yellow, the solution is still slightly acidic due to remaining TCA. Just add a small amount of 1 M Tris pH 8.0, until the color returns to the usual blue (do not use NaOH because you could hydrolyze the protein!).

Bromophenol Blue is an indicator used in chemistry that changes color from yellow to dark blue in the presence of free amino groups, allowing for noninvasive and continuous detection of these groups. AI generated definition based on: The Combinatorial Index, 1998

Bromophenol Blue is a sulfonated hydroxyquinone, tracking dye in DNA and RNA electrophoresis and a vital stain to probe the blood-brain barrier. Shop now at Sigma Aldrich

2025年1月20日 · Bromophenol Blue is a versatile compound widely used in scientific research as a pH indicator. Known for its ability to change color depending on the pH of the solution, it plays a critical role in various laboratory applications, including electrophoresis, titration, and …

2025年1月27日 · Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

A bromophenol is an organic compound consisting of hydroxyl groups and bromine atoms bonded to a benzene ring. They may be viewed as hydroxyl derivatives of bromobenzene , or as brominated derivatives of phenol .

Bromophenol blue, Tetrabromophenolsulfonephthalein, is an acid-base indicator whose useful range as an indicator lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible.