Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]

Confocal microscopy offers several advantages over conventional optical microscopy, including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens.

Confocal microscopy provides the ability to collect clear images from a thin section of a thick sample with low background and minimal out-of-focus interference. Optical sectioning is a common application in the biomedical sciences and has been useful for materials science as well.

In recent years, the confocal Laser Scanning Microscope (LSM) has become widely established as a research instrument. The present brochure aims at giving a scientifically sound survey of the special nature of image formation in a confocal LSM.

By eliminating unnecessary scattered light from outside the focal plane and suppressing stray light, confocal microscopy improves lateral resolution by 30% compared to ordinary optical microscopy. This produces images that are easy to see with less blurring.

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sections from thick specimens. The basic key ...

The system is composed of a a regular ßorescence microscope and the confocal part, including scan head, laser optics, computer.

A confocal microscope is an advanced optical imaging device designed to enhance the resolution and contrast of micrographs by eliminating out-of-focus light. Unlike traditional microscopes, it uses point illumination and a spatial pinhole to focus on a single plane of the specimen.

In recent years, the confocal Laser Scanning Micro-scope (LSM) has become widely established as a research instrument. The present brochure aims at giving a scientifically sound survey of the special nature of image forma-tion in a confocal LSM.

In recent years, the confocal Laser Scanning Microscope (LSM) has become widely established as a research instrument. The present brochure aims at giving a scientifically sound survey of the special nature of image formation in a confocal LSM.