Direct RNA sequencing (SQK-RNA004-XL) - nanoporetech.com
1 天前 · Unlike DNA, RNA is translocated through the nanopore in the 3'-5' direction. However, the basecalling algorithms automatically flip the data, and the reads are displayed 5'-3'. ... Low RNA integrity (RNA integrity number <9.5 RIN, or the rRNA band is shown as a smear on the gel)
北京林业大学林学院团队在《Developmental Cell》上发表研究成 …
1 天前 · 【北京林业大学】 近日,我校林学院森林学付玉杰教授团队在Cell子刊《 Developmental Cell 》上在线发表了题为《Nanopore RNA direct sequencing identifies that m6A modification …
Improvements in RNA and DNA nanopore sequencing allow for …
2 天之前 · We additionally leveraged the direct RNA nanopore sequencing data for the detection of RNA modifications, such as N 6-methyladenosine and pseudouridine, which play a role in …
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Whole genome sequencing of hepatitis B virus using tiled …
3 天之前 · The capture-Illumina and HEP-TILE-Nanopore pipelines had consensus sequencing accuracy of 100% in mock samples with known DNA sequences. ... We used carrier RNA as …
单细胞全长转录组测序 | 从原理到应用,一文读懂细胞转录的“完整 …
3 天之前 · 以自闭症、阿尔茨海默病等神经退行性疾病研究为例,运用 Nanopore 单细胞测序,能清晰呈现病变细胞中长链非编码 RNA(lncRNA)转录本的变化,它们可以通过与 DNA …
Another Nanopore: PolySeq - by Nava Whiteford
4 天之前 · PolySeq appear to be developing an Oxford Nanopore-like stranded protein nanopore DNA/RNA sequencing instrument. They show a device weighing 1.5Kg, with 2560 wells/pores …
Duplex PCR-Nanopore sequencing assay for - ScienceDirect
5 天之前 · Species and subtypes assessed by Sanger sequencing, were successfully detected using Nanopore sequencing with 95 % concordance. Further, sequence similarity was …
Gene expression regulation and polyadenylation in ulcerative …
5 天之前 · Ulcerative colitis (UC) is an immune-mediated chronic intestinal disease, with a pathogenesis that remains incompletely understood. The purpose of this study is to analyze …
Nanopore sequencing-based measurement of paramyxovirus RNA …
4 天之前 · Schematic diagram of RNA editing in the mRNA, antigenome and genome (A) Schematic diagram showing the viral RNA species in SeV-infected cells. Authentic mRNA …
Exploring Noncding RNAs Through Next-Generation Sequencing …
5 天之前 · 5.2.3.2 Oxford Nanopore Sequencing. The Oxford Nanopore sequencing (ONT) method was created to ascertain the nucleotide order of a DNA sequence. ... RNA-seq by …
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