2021年10月26日 · Here, we investigated how the NLRP3 inflammasome is regulated. We found that its activation in primary mouse macrophages induced the Src family kinase Lyn to phosphorylate NLRP3 at Tyr 918, which correlated with a subsequent increase in its ubiquitination that facilitated its proteasome-mediated degradation. NLRP3 tyrosine phosphorylation and ...

2018年10月31日 · Here we show that the Src-family kinases (SFKs)-Cbl axis plays a pivotal role in suppressing NLRP3 inflammasome activation in response to stimulation by nigericin or ATP, as assessed using gene...

2021年10月26日 · Here, we investigated how the NLRP3 inflammasome is regulated. We found that its activation in primary mouse macrophages induced the Src family kinase Lyn to phosphorylate NLRP3 at Tyr 918, which correlated with a subsequent increase in its ubiquitination that facilitated its proteasome-mediated degradation. NLRP3 tyrosine phosphorylation and ...

2024年12月25日 · Experiments in cell cultures and mice show that nintedanib inhibited NLRP3–IL-1β activation by Src/EGFR signaling. The discovery of this mechanism may lead to the novel access of the drug development for hepatic fibrosis in the future, and Src has the potential as a drug target for the treatment of hepatic fibrosis.

2017年12月18日 · Here we show NLRP3 inflammasome stimulators trigger Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2) translocation to the mitochondria, to interact with and...

2020年5月1日 · The NLRP3 inflammasome is a multi-protein complex that triggers the activation of inflammatory caspase-1 and the maturation of IL-1β in response ... Guoxin Lin, Hui Guo, Jian Zhang, Tyrosine Phosphorylation of NLRP3 by Src Family Protein Tyrosine Kinase Lyn Suppresses NLRP3 Inflammasome Activation, The Journal of Immunology, Volume 204, Issue ...

2020年5月1日 · Here, we show that NLRP3 is tyrosine phosphorylated upon activation of the NLRP3 inflammasome, and that NLRP3 tyrosine phosphorylation correlates with its ubiquitination. Further, we identified Lyn as the protein tyrosine kinase that phosphorylates NLRP3 at Tyr 918, which facilitates its ubiquitination and proteasome-mediated degradation.

2018年10月31日 · Here we show that the Src-family kinases (SFKs)-Cbl axis plays a pivotal role in suppressing NLRP3 inflammasome activation in response to stimulation by nigericin or ATP, as assessed using gene knockout and gene knockdown cells, dominant active/negative mutants, and pharmacological inhibition.

2023年3月15日 · In this study, we identified mitochondrial Src family kinases (mSFKs) as key determinants of GF-induced NLRP3 inflammasome activation. Comprehensive analysis of the TKIs revealed that all TKIs we tested act as potent agonists for the NLRP3 inflammasome in human monocytic THP-1 cells and bone marrow-derived macrophages.

2017年5月1日 · Pretreating the macrophages with a specific Src kinase inhibitor inhibits NLRP3 tyrosine phosphorylation and ubiquitination, which leads to enhanced production of IL-1β. Further we found that Src family PTK Lyn phosphorylates NLRP3 at Tyr918 which facilitates its ubiquitination and proteasome-mediated degradation.