Quantification of iNOS mRNA with reverse transcription
We report here a method for quantitative measurement of iNOS mRNA levels with rtPCR directly from cells lysed with a single step phenol/chloroform/ether extraction. Using a mouse …
Inducible Nitric Oxide Synthase: Regulation, Structure, and …
Many potent iNOS inhibitors with high selectivity over related NOS isoforms, neuronal NOS (nNOS) and endothelial NOS (eNOS), have been discovered, and these drugs have shown …
Regulation of human inducible nitric oxide synthase expression …
2019年7月1日 · siRNA-mediated down-regulation of Upf1 enhances cytokine-induced iNOS- mRNA- and protein-expression. DLD-1 cells were transiently transfected with a specific anti …
iNOS (NOS2)靶点介绍及实验小贴士| Abcam中文官网
内毒素、细胞因子、IFNG/ IFN-γ能与细菌脂多糖 (LPS)、TNF或IL1B/白细胞介素-1 β协同作用诱导iNOS的表达。 在肝脏、视网膜、骨细胞和肺的气道上皮细胞中表达。 在血小板中不表达。 …
细胞因子诱导型一氧化氮合酶（iNOS）在心肌细胞中的表达。体外单心脏心肌细胞中iNOS表达的表征和调节以及iNOS …
地塞米松和转化生长因子-β均减弱了il-1β和infγ诱导的inos mrna丰度和酶活性。 地塞米松的预处理也消除了iNOS mRNA的诱导，但没有消除注射了脂多糖的大鼠的纯化心肌细胞中GTP环水解 …
一氧化氮合酶表达的调节 - 知乎 - 知乎专栏
在培养的牛主动脉内皮细胞，TNFa可下调 eNOS mrNA、酶蛋白及其活性，但不引起iNOS平行诱导，提示下调eNOS表达和诱导iNOS表达可独立出现。 TNF-a可降低 eNOS mRNA稳定性， …
Regulation of the expression of inducible nitric oxide synthase
2010年9月15日 · Increased gastric iNOS mRNA expression was found in subjects with long CCTTT-repeats (either allele >11) in the iNOS promoter analyzing H. pylori-infected patients …
Demonstration of iNOS-mRNA and iNOS in human monocytes
Synthesis and localization of inducible nitric oxide synthase mRNA (iNOS-mRNA) and iNOS protein in the cultures of human monocytes (Mphi) and colon carcinoma cell line (DeTa) that …
Human Mononuclear Phagocyte Inducible Nitric Oxide Synthase (iNOS …
1995年8月1日 · By reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, we show that human monocytes can be induced to express iNOS mRNA after treatment with …
Human mononuclear phagocyte inducible nitric oxide synthase (iNOS …
1995年8月1日 · The purpose of this study was to determine if stimulated human mononuclear phagocytes produce inducible nitric oxide synthase (iNOS) mRNA, protein, and enzymatic …