Polymerisation of host cell actin into “actin tails” propels B. pseudomallei to the membranes of host cells where the type VI secretion system fuses host cells into multinucleated giant cells (MNGCs) to facilitate cell-to-cell dissemination. This review describes the various mechanisms used by B. pseudomallei to survive within cells.

2023年10月9日 · It is a Gram-negative rod-shaped bacterium measuring about 2 – 5 μm in length and about 0.4 – 0.8 μm in width. It shows bipolar staining and gives its characteristic ‘safety pin’ appearance under a microscope. Burkholderia pseudomallei. Image Source: Public Health Image Library (PHIL), CDC.

2006年4月1日 · Melioidosis is caused by the aerobic, Gram-negative soil-dwelling bacillus Burkholderia pseudomallei and is an important cause of severe sepsis in Southeast Asia and Northern Australia.

Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens. Am J Trop Med Hyg 89:165–168. doi: 10.4269/ajtmh.13-0066. [ DOI ] [ PMC free article ] [ PubMed ] [ Google Scholar ]

Therefore, this review aims to frame the present knowledge of a potential target known as the Burkholderia invasion protein D (BipD), including future directions for its detection using an aptamer-based sensor (aptasensor). Keywords: melioidosis, B. pseudomallei, BipD, aptasensor. 1. …

Microscopy and Staining. B. pseudomallei is a Gram-negative bacillus that typically exhibits a bipolar or safety pin appearance, which is better appreciated when stained with methylene blue.

2023年4月5日 · In this study, we performed comparative transcriptome analysis of B. pseudomallei cultured in human plasma versus soil extract media to provide insights into B. pseudomallei gene expression that governs bacterial adaptation and infectivity in the host.

Polymerisation of host cell actin into “actin tails” propels B. pseudomallei to the membranes of host cells where the type VI secretion system fuses host cells into multinucleated giant cells (MNGCs) to facilitate cell-to-cell dissemination. This review describes the various mechanisms used by B. pseudomallei to survive within cells.

The occurrence of latency and relapse in human melioidosis suggests adaptations by Burkholderia pseudomallei that help to avoid the human immune response. Ruthenium red-stained preparations of bacterial cultures viewed by electron microscopy revealed three morphologically distinct variants; one with …

2021年5月26日 · Overall, we show how a Burkholderia -specific phage tail fiber can be exploited for rapid detection of B. pseudomallei. The 94TF-LAA has the potential for further development as a supplementary diagnostic to assist in clinical identification of this life-threatening pathogen.