Peptide Synthesis - history, protecting groups, reagents, practical ...
2023年12月25日 · The pioneering work of Bruce Merrifield, which introduced solid phase peptide synthesis (SPPS), dramatically changed the strategy of peptide synthesis and simplified the tedious and demanding steps of purification associated with solution phase synthesis.
26.7: Merrifield Solid-Phase Peptide Synthesis
This procedure, known as the Merrifield Synthesis after its inventor R. Bruce Merrifield, involves attaching the C-terminus of the peptide chain to a polymeric solid, usually having the form of very small beads. Separation and purification is simply accomplished by filtering and washing the beads with appropriate solvents.
26.9: The Merrifield Solid-Phase Technique - Chemistry LibreTexts
This procedure, known as the Merrifield Synthesis after its inventor R. Bruce Merrifield, involves attaching the C-terminus of the peptide chain to a polymeric solid, usually having the form of very small beads. Separation and purification is simply accomplished by filtering and washing the beads with appropriate solvents.
26.8Automated Peptide Synthesis: The Merrifield Solid-Phase …
In the Merrifield solid-phase method, peptide synthesis is carried out with the growing amino acid chain covalently bonded to small beads of a polymer resin, rather than in solution. In the original procedure, polystyrene resin was used, prepared so that one of every hundred or so benzene rings contained a chloromethyl (−CH 2 Cl) group.
26.8: Automated Peptide Synthesis- The Merrifield Solid-Phase …
2024年9月30日 · The Merrifield solid-phase method is a revolutionary technique for automated peptide synthesis. This method involves attaching the first amino acid to a solid support and sequentially adding …
Merrifield Solid-Phase Peptide Synthesis - Chem-Station Int. Ed.
2014年2月2日 · Solid phase peptide synthesis (SPPS), developed by R. B. Merrifield, was a major breakthrough allowing for the chemical synthesis of peptides and small proteins. SPPS results in high yields of pure products and works more quickly than classical synthesis (liquid-phase peptide synthesis, LPPS).
Merrifield Solid-Phase Peptide Synthesis Explained ... - Pearson
The Merrifield solid phase peptide synthesis method involves four key steps: amino acid protection, attachment to a chloromethylated polystyrene bead via an SN2 reaction, hydrolysis to deprotect the amino group, and peptide chain release using hydrofluoric acid.
Solid phase peptide synthesis - ScienceDirect
2023年1月1日 · In 1963, R.B. Merrifield reported a novel strategy of solid phase peptide synthesis (SPPS) (Merrifield, 1963). The advent of SPPS has brought a revolution in the peptide synthesis. Merrifield was awarded Nobel Prize in Chemistry in 1984 …
Solid Phase Synthesis (Nobel Lecture) - Merrifield - 1985
A solid phase as “protecting group” in peptide synthesis—this was the original idea of Bruce Merrifield, who received the 1984 Nobel Prize for Chemistry. In his lecture, he describes the development of the Merrifield synthesis.
Merrifield Solid-Phase Peptide Synthesis - Wiley Online Library
2010年9月15日 · The synthesis of peptides and small proteins in which the resinous polymer supported amino acid and succeeding peptide repeatedly reacts with N -protected amino acids followed by deprotection until the desired peptide or protein is assembled is generally referred to as the Merrifield solid phase peptide synthesis (or SPPS) and the polymeric resi...
- 某些结果已被删除