2025年1月1日 · Self-sustained sequence replication (3SR): an isothermal transcription-based amplification system alternative to PCR PCR Methods Appl . 1991 Aug;1(1):25-33. doi: 10.1101/gr.1.1.25.

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3SR reaction. (14) Such developments include: (1) optimization of substrate concentrations, (2) the effect of temperature, pH, and ionic strength on amplification productivity, and (3) the reaction conditions required that enable the 3SR reaction to be carried out with only AMV RT and T7 RNA

One of these methods is the self-sustained sequence replication (3SR) reaction, which is an isothermal method for RNA amplification depending on the action of three enzymes. 3SR has been used in several in vitro applications and has also been modified for in situ use (IS-3SR).

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2025年3月1日 · Sr-doped γ - Ga 2 O 3 achieves stable and efficient photocatalytic overall water splitting. Sr doping weakens the Ga-OO bond, limiting surface peroxidation and reducing the O 2 desorption energy. Rh/Cr 2 O 3 improves charge separation and suppresses the reverse reaction of water splitting.

To minimize the manipulations required for in vitro selection, we chose to use self-sustained sequence replication (3SR) [28,29] to amplify RNAs that are isolated during each round of selection. 3SR is an isothermal amplification technique that utilizes two enzymes to exponentially amplify both DNA and RNA in a single reaction mixture.