The Argonaute (AGO) gene family encodes six characteristic domains: N- terminal (N), Linker-1 (L1), PAZ, Linker-2 (L2), Mid, and a C-terminal PIWI domain. [8]

2024年7月30日 · Argonaute 2蛋白 （AGO2），亦称 真核翻译起始因子2C2 ( eukaryotic translation initiation factor 2C subunit 2，EIF2C2) 。含有N末端( amino-terminal) 、PAZ、MID 和PIWI 四个球状结构域，以及L1、L2 两个连接域，属于Argonaute 蛋白(AGO)家族成员。

2024年12月24日 · AGO2 (Argonaute RISC Catalytic Component 2) is a Protein Coding gene. Diseases associated with AGO2 include Lessel-Kreienkamp Syndrome and Non-Specific Syndromic Intellectual Disability. Among its related pathways are Transcriptional Regulation by MECP2 and Cell junction organization.

2023年10月16日 · AGO2（Argonaute 2）蛋白是RNA干扰（RNAi）和微小RNA（miRNA）通路中的一种关键因子，具有重要的生物学功能。 具体来说，AGO2蛋白可与小RNA（如siRNA、miRNA等）配对，并沟通RNA干扰途径和翻译后调节途径，参与基因表达调控、DNA修复、免疫防御等多种生物过程。

2020年11月16日 · We identify 13 heterozygous mutations in AGO2 in 21 patients affected by disturbances in neurological development. Each of the identified single amino acid mutations result in impaired...

Ago2也是四种Ago蛋白中表达量最高的一种，诸多实验证明，Ago2就是Slicer酶，活性依赖于二价金属阳离子。 所有四种人类Ago的效应复合物都会引起翻译抑制和mRNA降解。

The human Argonaute2 protein (Ago2) is a key player in RNA interference pathway and small RNA recognition by Ago2 is the crucial step in siRNA mediated gene silencing mechanism. The present study highlights the structural and functional dynamics of human Ago2 and the interaction mechanism of Ago2 with a set of seven siRNAs for the first time.

2020年11月19日 · Argonaute (AGO) 蛋白是 RNA 介导的转录后基因调控的关键蛋白，为 miRNA 提供锚位点，形成 RNA 诱导沉默复合体 RISC 达到降解靶基因或者抑制翻译的目的。 自 1993 年 miRNAs 被发现以来，研究人员已经积累了数百种不同 miRNA 分子及其生成、成熟在发育、生理和疾病中的作用机制。 尽管人类早已明确 miRNA 半衰期非常短，细胞在使用完毕后如何处理（降解） miRNA，精确调控 miRNA 周转机制的蛋白和执行策略知之甚少。 近三十多年来，研究 …

Argonaute2蛋白 （AGO2）通过参与构成 RNA诱导的沉默复合体,其自身催化及自身过表达功能,在人类肿瘤的发生发展中扮演了重要角色,逐渐成为研究焦点。 Ago2表达水平变化或功能失调时，可作为抑癌或 致癌基因 参与多种肿瘤的发生、发展，与肿瘤细胞的增殖与分化 ...

AGO2 is the only member with catalytic activity in Argonaute family and contains four functional core domains, which are N domain, PAZ domain, MID domain, and PIWI domain from N-terminal to C-terminal. In traditional view, AGO2 serves as the catalytic engine of the RNA induced silencing complex and …

2021年6月11日 · Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures...

2025年2月20日 · AGO2 Mediates MYC mRNA Stability in Hepatocellular Carcinoma. Human Argonaute2 and Argonaute3 are catalytically activated by different lengths of guide RNA. AGO2 and its partners: a silencing complex, a chromatin modulator, and new features. Germline AGO2 mutations impair RNA interference and human neurological development.

Mohamed et al. report the cryoelectron microscopy structure of human AGO2 with fully paired guide RNA. Their analysis reveals the structural basis for the slicing activity that drives RNAi, showing that the slicing-competent conformation is achieved by domain movements and RNA-protein contacts distinct from those of conformational intermediates ...

2023年8月15日 · Argonaute 2 (Ago2) is a key component of the RNA interference (RNAi) pathway, a gene-regulatory system that is present in most eukaryotes. Ago2 uses microRNAs (miRNAs) and small interfering RNAs (siRNAs) for targeting to homologous mRNAs which are then degraded or translationally suppressed.

近日，Whitehead研究所 David P. Bartel 研究组在 Science 发表文章 The biochemical basis of microRNA targeting efficacy，对RNA bind-n-seq（RBNS）测序进行优化，用于 测量miRNA-AGO复合体与所有小于12nt靶标结合的相对亲和力的大小，为定量测量miRNA对于基因调控网络的结合效率提供了重要的生物化学基础。 靶标结合的效率是miRNA-AGO复合体与靶标序列之间的亲和力的大小，在这种情况下，与目标位点的更大亲和力将导致该位点的占用率增加，从而增加 …

2024年7月31日 · Argonaute 2蛋白（AGO2），亦称真核翻译起始因子2C2 ( eukaryotic translation initiation factor 2C subunit 2，EIF2C2) 。 含有N末端( amino-terminal) 、PAZ、MID 和PIWI 四个球状结构域，以及L1、L2 两个连接域，属于Argonaute 蛋白(AGO)家族成员。

2020年3月13日 · In traditional view, AGO2 serves as the catalytic engine of the RNA induced silencing complex and plays an important role in small RNAs guided post transcriptional gene silencing, including mRNA degradation and translational repression.

Argonaute 2 (Ago2) is a key component of the RNA interference (RNAi) pathway, a gene-regulatory system that is present in most eukaryotes. Ago2 uses microRNAs (miRNAs) and small interfering RNAs (siRNAs) for targeting to homologous mRNAs which are then degraded or translationally suppressed.

2024年7月31日 · Argonaute 2蛋白（AGO2），亦称真核翻译起始因子2C2 ( eukaryotic translation initiation factor 2C subunit 2，EIF2C2) 。 含有N末端( amino-terminal) 、PAZ、MID 和PIWI 四个球状结构域，以及L1、L2 两个连接域，属于Argonaute 蛋白(AGO)家族成员。

We identify 13 heterozygous mutations in AGO2 in 21 patients affected by disturbances in neurological development. Each of the identified single amino acid mutations result in impaired shRNA-mediated silencing. We observe either impaired RISC formation or increased binding of AGO2 to mRNA targets as mutation specific functional consequences.