AuTophaGy related 1 (Atg1) is a 101.7kDa serine/threonine kinase in S.cerevisiae, encoded by the gene ATG1. [1] It is essential for the initial building of the autophagosome and Cvt vesicles. In a non-kinase role it is - through complex formation with Atg13 and Atg17 - directly controlled by the TOR kinase , a sensor for nutrient availability.

Atg1(ULK1)蛋白激酶复合体. Atg1是第一个在酵母中被成功克隆的自噬基因,哺乳动物中的同源基因为ULK1 (unc-51-like kinase 1)。ULK1是自噬泡形成所必需的一种蛋白，以复合物的形式存在, 与至少三种蛋白质组成复合物：ATG13，FIP200和ATG101。

Here, we investigated the role(s) of the Arabidopsis (Arabidopsis thaliana) ATG1 kinase during autophagy through an analysis of a quadruple mutant deficient in all four ATG1 isoforms.

2025年2月21日 · ATG1 stimulates autophagy biogenesis and serves as a gatekeeper for classical autophagy. To obtain insight into the control of autophagy by ATG1 and determine whether ATG1 has broader processes, we performed a thorough proteomics analysis on the Col-0 wild-type and atg1abct mutant in Arabidopsis thaliana.

ULK1、 ULK2 与一种自噬相关 (Atg) 基因产物 (mAtg13) 的哺乳动物同源体，以及骨架蛋白 FIP200（与酵母 Atg17 直接同源）共同形成一个较大的复合体。 III 级 PI3K 复合体，包含 hVps34、 Beclin-1（酵母 Atg6 的一种哺乳动物同源体）、p150（酵母 Vps15 的一种哺乳动物同源体）以及 Atg14 样蛋白 (Atg14L 或 Barkor) 或抗紫外辐射相关基因 (UVRAG)，为诱导自噬所必须。 Atg 基因通过 Atg12-Atg5 以及 LC3-II (Atg8-II) 复合体，控制自噬体的形成。 Atg12 通过 …

ATG1 encodes a protein kinase, and the catalytic activity of Atg1 is essential for autophagy (19, 28). Atg1 forms a complex with several proteins, including Atg11, Atg13, Atg17, Atg29, and Atg31 (5, 16, 19, 21, 22). Atg13 is highly phosphorylated in a TORC1-dependent manner under nutrient-rich conditions, and phosphorylated Atg13 does not ...

Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in CC4 domain of Atg11.

Here, we describe the ATG1/13 complex in Arabidopsis thaliana and show that it is both a regulator and a target of autophagy. Plants missing ATG13 are hypersensitive to nutrient limitations and senesce prematurely similar to mutants lacking …

2020年9月24日 · 从酵母到哺乳动物都保守的一系列自噬相关蛋白（Atg proteins）在自噬过程中发挥关键作用。其中，Atg1（哺乳动物中称为ULK1）是一个丝氨酸/苏氨酸激酶，其激酶活性对于自噬是必需的。Atg1活性的调控机制之前仅在芽殖酵母Saccharomyces cerevisiae中有较深入的研究。

2020年5月5日 · The Atg1/ULK complex is an initiator kinase complex for autophagy that serves as a scaffold to recruit downstream factors and regulate their functions via phosphorylation of serine or threonine...