2024年6月10日 · In single-transfection experiments at safe-harbour and therapeutically relevant sites, PASSIGE with eeBxb1 led to an average targeted-gene-integration efficiencies of 23% (4.2-fold that of...

We built and characterized a novel recombinase mediated cassette exchange (RMCE) system using Bxb1 integrase and compared it to the commonly used Flp/FRT RMCE system.

2022年3月31日 · Bxb1 Int-mediated recombination uses a minimal attachment site of 48 bp attP (attachment Phage) and a cognate 38 bp site attB (attachment Bacterial) between the donor and recipient DNAs. The ...

2012年3月21日 · The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and mammalian cells.

2017年1月1日 · Here, we introduce Bxb1 integrase as a more efficient candidate for joining multiple DNA molecules. Bxb1 integrase-mediated highly efficient metabolite pathway assembly with a compatible BioBrick standard. The standardization of biological parts and their assembly is one of the core ideas in synthetic biology.

2013年10月20日 · Bxb1 integrase yielded approximately two-fold more recombinants and displayed about two fold less damage to the recombination sites than the next best recombinase; φC31 integrase.

2024年10月21日 · To address these issues, we developed Bxb1 variants that demonstrate increased specificity and potency in vitro and have engineered stabilized Bxb1 variants that increase in vivo activity over 25-fold enabling targeted …

2023年11月3日 · Here, we show that a point mutation (Bxb1-GA) in Bxb1 target sites significantly increases Bxb1-mediated integration efficiency at the Rosa26 locus in Chinese hamster ovary cells, resulting in the highest integration efficiency reported with a site-specific integrase in mammalian cells.

2 天之前 · Bxb1 integrase, a serine recombinase from mycobacteriophage Bxb1, is widely used for precise genetic modifications. Its efficiency in site-specific recombination allows for the stable integration of large DNA sequences into target genomes with minimal off-target effects, making it valuable in gene therapy, synthetic biology, and biotechnology.

2023年11月17日 · Here, we harnessed two orthogonal Bxb1 recombinase sites to enable alternative avenues for using mammalian synthetic biology to characterize transgenic protein variants.