What is the difference between sequence, reads, and contigs of …
2015年5月19日 · My understanding of those three words as follows: sequence is a generic name describing order of biological letters (DNA/RNA or amino acids). Both contigs and reads are …
dna sequencing - What is the difference between contig- and read …
2017年6月1日 · Is contig based alignment refer to de novo assembly and then it is align to a reference genome. I am confused that if you have read based alignment then what is the …
An example for N50? Why do we need it? - Biology Stack Exchange
2015年5月9日 · Contig or scaffold N50 is a weighted median statistic such that 50% of the entire assembly is contained in contigs or scaffolds equal to or larger than this value. Mathematically: …
dna sequencing - What is the difference between sequence …
2015年11月9日 · Assembling means that you take a bunch of short sequences and try to stitch them back together to reconstitute the original DNA sequence. Fig1: Screen capture from DNA …
Understanding Contig NG50 - Biology Stack Exchange
2021年7月3日 · Contig NG50 is defined as the length at which half of the predicted genome size is contained in contigs longer than this length. I can’t understand this and googling returned …
What is "contigs" in Picard's ReorderSAM? - Biology Stack Exchange
ReorderSam reorders reads in a SAM/BAM file to match the contig ordering in a provided reference file, as determined by exact name matching of contigs Q: What does contig ordering …
What's the difference between shotgun sequencing and clone …
In short: the basis of the process is the same in both, the difference relies that in the clone based sequencing you make DNA library of the pieces of DNA clones you got from the sequence you …
How to identify an unknown species from its genome sequence
2020年10月6日 · I am now interested in using the genetic sequences from my contigs to identify the original organism (I know it is a bacterium). I have tried using ncbi's BLASTn on one of my …
How to annotate a .broadpeak file with 9 columns?
2015年11月21日 · This is what the columns of broadPeak format mean (from UCSC): chrom - Name of the chromosome (or contig, scaffold, etc.). chromStart - The starting position of the …
What exactly are computers used for in DNA sequencing?
As a consequence, you instead end up with distinct, contiguous blocks (“contigs”) of mapped reads. Each contig is a sequence fragment, like reads, but much larger (and hopefully with …