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Cytospin preparation technique - EuroMAbNet
A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.
20191024-Cytospin离心和直接涂片的区别 - 哔哩 ... - 哔哩哔哩
cytospin离心条件:200rpm, 5min, 细胞浓度1X10^6/ml, 分别加20ul, 50ul, 100ul, 200ul。 离心结束后,甲醇固定及染色步骤与涂片相同。 下图为20ul和200ul的结果(figure1)。 Figure1. 镜下的结果(cytospin离心) 2. 直接涂片. 用黄枪头吸10ul,20ul细胞悬浮液,直接均匀涂抹于载玻片上(下次不用涂那么开),等待15-20min,直到液体完全干。 固定及染色步骤与cytospin离心后的步骤相同。 下图为结果(figure2)。 Figure2.镜下的结果(涂片) 二、总结.
Thermo Scientific Shandon Cytospin Collection Fluid Instructions for Use For in vitro diagnostic use. For fixation of cytology specimens. Most Cytotechnologists would prefer to work with fresh unfixed specimens. Unfortunately, cytology specimens are often collected at a distant location and later transported to the laboratory.
赛默飞细胞离心涂片制备整体解决方案 - 分析测试百科网
2019年5月23日 · 赛默飞Cytospin 4细胞离心涂片机是第4代的细胞离心涂片系统,具有操作简单易行、职业安全性高、样本安全性高、富集细胞、制备质量高等优势,在国内外广泛应用;传统的手工涂片法,在样本制备过程易出现细胞挤压变形、细胞丢失严重导致数量少、有重叠及 ...
Shandon Cytospin 4 细胞离心涂片机 - ndchina.com.cn
2014年3月24日 · 40 余年来, Thermo Scientific Cytospin 作为细胞离心涂片机的代名词,为全世界提供了经济可靠的液基样本的单层细胞制作方案。 产品概览 ·适用于所有液基样本,尤其是细胞量含量低的液基样本,如脑脊液、尿液
The Shandon Cytospin has been synonymous with cytocentrifugation for over 40 years. More than 15,000 laboratories worldwide use this simple, reliable instrument. It provides economical thin-layer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine. The Shandon Cytospin was the first device to use
Cytospin methanol fixed human white blood cells on microdissection slide. A drop of fixed cells is pipetted into 1 ml of 50% acetic acid (to remove cytoplasmic proteins) contained in a specially designed centrifuge
Thermo ScientificTM ShandonTM CytoblockTM Cell Block Preparation System is used in conjunction with the Thermo Scientific Cytospin and facilitates processing of FNA’s, tissue fragments and cell buttons.
Designed for ease of use and eficiency, the single-use Thermo ScientificTM ShandonTM EZ CytofunnelTM is an ideal solution for today’s busy laboratories. To use, insert the Thermo ScientificTM ShandonTM CytoslideTM into the EZ Cytofunnel. Twelve EZ Cytofunnel assemblies fit into the Cytospin 4 head.
Thermo Shandon Cytospin 4 细胞离心涂片机 - 生物器材网
2024年12月5日 · Shandon Cytospin 是第一款使用精密控制的离心技术对细胞进行分离,形成薄层同时还能保持细胞完整的设备。 它采用离心原理,将细胞 转移到诊断载玻片的固定区域,样品残液则被过滤卡吸收。
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