2023年8月9日 · 共同伴侣辅助蛋白（DnaJ/Hsp40）刺激ATP水解为ADP（黄色），导致底物结合域发生巨大的构象变化，导致其构象闭合，底物被锁定在底物结合域；这里促进了适当的折叠（步骤2和步骤3）。 在其他辅助伴侣蛋白（GrpE/BAG1）的刺激下，用ATP交换结合的ADP，将Hsp70转化回开放构象（第4步），释放适当折叠的底物（第5步），再生开放构象，然后与其他底物相互作用。 (b)二聚体Hsp90分子伴侣的三种构象状态，被认为参与了底物重构。

2012年3月9日 · Upon deletion of TF, DnaK interacts increasingly with ribosomal and other small, basic proteins, while its association with large multidomain proteins is reduced. DnaK also functions prominently in stabilizing proteins for subsequent folding by GroEL.

The 70 kilodalton heat shock proteins (Hsp70s or DnaK) are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms.

2012年3月29日 · DnaK, the major bacterial Hsp70, is one of the most abundant constitutively expressed and stress inducible chaperones in the E. coli cytosol. Yet it is not essential under nonstress conditions at intermediate temperature (Bukau and Walker, 1989).

2020年1月17日 · The ATP-dependent Hsp70 chaperones (DnaK in E. coli) mediate protein folding in cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE, respectively).

DnaJ proteins are molecular co-chaperones that recruit DnaK family chaperones (heat shock family) to perform functions such as protein folding, protein transport, or remodeling of protein complexes. The energy released from ATP hydrolysis by …

2024年1月22日 · Here we report a cryo-EM analysis of GrpE bound to an intact Mtb DnaK, revealing an asymmetric 1:2 DnaK−GrpE complex. The GrpE dimer ratchets to modulate both DnaK nucleotide-binding domain...

2022年6月16日 · Our results demonstrate that the initial DnaK response is largely dependent on protein synthesis rate but as the recombinantly expressed protein accumulates and homeostasis is approached the...

We investigated the interaction of the major inducible hsp from mammalian (Hsp70) and bacterial (DnaK) species with liposomes. We found that mammalian Hsp70 displayed a high specificity for negatively charged phospholipids, such as phosphatidyl serine, whereas DnaK interacted with all lipids tested regardless of the charge.

来自大肠杆菌的热休克蛋白DnaK，DnaJ和GrpE（KJE）构成三组分分子伴侣系统，该分子伴侣系统可防止变性蛋白质聚集并以ATP依赖性方式协助蛋白质重新折叠。