The epitope EFRH, corresponding to amino acids 3–6 within the human β-amyloid peptide (AβP), acts as a regulatory site controlling both the formation and disaggregation process of the β-amyloid fibrils (Aβ).

In the present study we have found that phage displaying high EFRH copy number are effective in eliciting humoral response against the EFRH sequence, which in turn relieves the amyloid burden in the brains of amyloid precursor protein Tg mice and improves their ability to …

2000年10月10日 · The epitope EFRH, corresponding to amino acids 3–6 within the human β-amyloid peptide (AβP), acts as a regulatory site controlling both the formation and disaggregation process of the β-amyloid fibrils (Aβ).

1998年8月1日 · Among 44 positive phage-clones, selected from the library by both antibodies, 40 clones carried the consensus sequence EFRH. These EFRH phage-clones bind specifically mAbs 6C6 or 10D5 with an apparent binding constant of approximately 10 (-9) M.

1998年8月1日 · These findings confirm that the peptide EFRH which is located at positions 3–6 within β -amyloid peptide represents the sequential epitope of mAbs 6C6 and 10D5. 1. Introduction. One of the major histopathological hallmarks of Alzheimer's disease (AD) is the abundant presence of amyloid plaques in the brain tissues of affected individuals.

The epitope EFRH, corresponding to amino acids 3-6 within the human beta-amyloid peptide (AbetaP), acts as a regulatory site controlling both the formation and disaggregation process of the beta...

2007年1月30日 · Here we show that anti-EFRH immunotherapy of a platelet-derived amyloid precursor protein transgenic mouse model of AD stimulates endogenous neurogenesis, suggested by elevated numbers of BrdU-incorporated cells, most of which are colocalized with a marker of mature neurons, NeuN.

2000年10月10日 · The epitope EFRH, corresponding to amino acids 3-6 within the human beta-amyloid peptide (AbetaP), acts as a regulatory site controlling both the formation and disaggregation process of the beta-amyloid fibrils (Abeta). Locking of this epitope by highly specific antibodies affects the dynamics of th …

1999年3月1日 · The EFRH peptide strongly inhibits binding of mAbs 6C6 and 10D5 to βAP, whereas it inhibits weakly the interaction of 2H3 with βAP. Low affinity binding of mAb 2H3 to EFRH might explain its failure in prevention of β-amyloid formation.

We have developed an immunization procedure for the production of effective anti-beta-amyloid (anti-Abeta) antibodies, using filamentous phage displaying only 4 amino acids. The EFRH sequence, encompassing amino acids 3-6 of the 42 residues of Abeta peptide, was found previously to be the main regul …