FITC is a fluorescein derivative consisting of a fluorescein core and an amine-reactive isothiocyanate moiety. The isothiocyanate group reacts with primary amines forming covalent thiourea bonds that attach fluorescein to the biomolecule.

Fluorescein (FITC) derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water-solubility. Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications.

FITC，英文全称 Fluorescein Isothiocyanate，中文名称异硫氰酸荧光素，是生物学中应用最为广泛的一种绿色荧光素衍生物。 FITC 具有高吸收率和优良的荧光量子产率等特点。 FITC 的异硫氰酸基团可与蛋白质上的氨基、巯基、咪唑、酪氨酰、羰基等基团相结合，从而实现包括抗体，凝集素在内的蛋白标记。 FITC 除了用作蛋白质标记物，还可用作蛋白质荧光示踪剂，通过标记抗体 …

FITC can be easily conjugated with antibodies against various tags. It also enables highly targeted detection when coupled with avidin or streptavidin, as both, along with biotin, can be linked to FITC through their available amine variants.

2009年4月18日 · Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield.

2022年3月17日 · First termed in 1942 by A.H. Coons, FITC is a unique fluorescein molecule having an isothiocyanate reactive group (-N=C=S), which replaced the hydrogen atom located at the base of its structure. In this article, we’ll discuss FITC labeling techniques and provide tips and tricks for maximizing yield.

FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry.

FITC fluorescein isothiocyanate | C42H22N2O10S2 | CID 131730176 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm. It is widely used to attach a fluorescent label to proteins via the amine group.