We characterized the protein encoded by FUM3, a gene in the fumonisin biosynthetic gene cluster. The 33-kDa FUM3 protein (Fum3p) was heterologously expressed and purified from Saccharomyces cerevisiae. Yeast cells expressing the Fum3p converted FB 3 to FB 1, indicating that Fum3p catalyzes the C-5 hydroxylation of fumonisins.

2025年2月20日 · Fumarate hydratases are tricarboxylic-acid cycle enzymes that catalyze the reversible interconversion of fumarate and malate. (Adapted from FBrf0030365). Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fum3p，一种在枯萎镰刀菌中伏马毒素的C-5羟化反应所需的2-酮戊二酸依赖性双加氧酶。 伏马菌素是由几种农业上重要的镰刀菌属物种生产的聚酮化合物衍生的霉菌毒素。 B系列伏马菌素FB（1），FB（2），FB（3）和FB（4）是由野生型镰刀菌（Fusarium v erticillioides）菌株产生的伏马菌素，其碳原子上附着的羟基数量和位置不同骨干。 我们表征了由伏马菌素生物合成基因簇中的一个基因FUM3编码的蛋白质。 33-kDa FUM3蛋白（Fum3p）从酿酒酵母中异源表达并 …

Confocal microscopy for localization of Fum17-GFP and Fum18-DsRed (A), Cer1-BFP (B), DsRed-Fum8 (C), and Fum3-GFP (D). Conidia were inoculated in ICI/6 mM Gln and grown as a standing culture...

Fusarium proliferatum is a common hemi-biotrophic pathogen that infect a wide range of host plants, often leading to substantial crop loss and yield reduction. F. proliferatum synthesizes...

Fumonisins are a group of mycotoxins produced by Fusarium verticillioides (synonym Fusarium moniliforme Sheldon). Fumonisin B1 has acute toxicity and potential carcinogenicity to some animals, causing leukoencephalomalacia in horses, …

2004年4月1日 · We characterized the protein encoded by FUM3, a gene in the fumonisin biosynthetic gene cluster. The 33-kDa FUM3 protein (Fum3p) was heterologously expressed and purified from Saccharomyces cerevisiae.

Gene ID: 39281, updated on 29-Dec-2023. Predicted to enable fumarate hydratase activity. Predicted to be involved in fumarate metabolic process; malate metabolic process; and tricarboxylic acid cycle. Predicted to be part of tricarboxylic acid cycle enzyme complex. Predicted to be active in mitochondrion.

We characterized the protein encoded by FUM3, a gene in the fumonisin biosynthetic gene cluster. The 33-kDa FUM3 protein (Fum3p) was heterologously expressed and purified from Saccharomyces cerevisiae. Yeast cells expressing the Fum3p converted FB (3) to FB (1), indicating that Fum3p catalyzes the C-5 hydroxylation of fumonisins.

2012年11月15日 · To our knowledge, this is the first report of the effects of a w on FUM3, FUM8, FUM13 and FUM14 expression in F. verticillioides. We have also demonstrated the effects of a w on bikaverin production and the expression of BIK1 and also confirmed the production of bikaverin under fumonisin inducing conditions.