H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear.

组蛋白H2AX是H2A的变体，在核小体中取代传统的H2A。 紫外线灯等辐射可导致 DNA 损伤，在损伤后几分钟内，组蛋白H2AX的Ser139 位点会发生快速磷酸化。 因此，第139位丝氨酸磷酸化的H2AX，被命名为gamma H2A.X，已被广泛用作DNA双链断裂（DSBs）的标记，来指示双链DNA损伤和凋亡。 在G1和S期表达。 定位在细胞核。 图二：gamma H2A.X ICC实验结果图， Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab195188)。 …

2010年2月4日 · Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA double-strand breaks. Detection of this...

2024年6月1日 · H2AX is a well-known chromatin modification event in DNA damage response and human malignancies. H2AX phosphorylation (γ-H2AX) is necessary for DNA repair protein assembly at damaged chromatin regions. Detecting double strand breaks with phosphorylated histone H2AX may help in cancer treatment.

γH2AX是DNA 损伤尤其是DNA 双链断裂(DNA double-strand breaks,DSB) 或DNA修复的标志物,已经被应用到医学相关领域,如放射性DNA 损伤的检测、癌症的辅助诊断以及预后监测。 此外,γH2AX在检测生殖细胞中的DNA 损伤及修复和维持胚胎干细胞的自我更新中有重要意义。 检测γH2AX水平已成为评价生殖细胞和干细胞质量的重要方法。 本文将从H2AX 及其家族的生物学特征、γH2AX 的检测方法、DNA损伤与修复中的H2AX 磷酸化及其在生殖相关细胞中的应用等角 …

组蛋白2A变异体（H2AX）在DNA双链断裂损伤时可以发生不同的翻译后修饰，包括磷酸化、乙酰化、甲基化和泛素化。 当细胞发生DNA损伤时，H2AX的氨基酸保守序列发生磷酸化。 早有研究表明，H2AX是最早被磷酸化的底物，是细胞中感应DNA损伤最敏感的分子。 γ-H2AX焦点的数量和大小可以通过免疫荧光或细胞流式技术检测得到，且数量和大小与一定范围的损伤剂量相关。 所以，越来越多的研究提出γ-H2AX是DNA损伤的一个很好的蛋白标记物。 03\ TUNEL检测 —基 …

碧云天生产的DNA损伤检测试剂盒(γ-H2AX免疫荧光法)，英文名为DNA Damage Assay Kit by γ-H2AX Immunofluorescence，是通过免疫荧光染色检测DNA损伤标记物γ-H2AX (即磷酸化的H2AX)的含量以确认DNA是否损伤的检测试剂盒。

H2AX is a histone H2A variant that constitutes 2–25% of mammalian histone H2A depending on the organism and cell type (Redon et al. 2002; Rogakou et al. 1998).

2024年12月25日 · H2AX (H2A.X Variant Histone) is a Protein Coding gene. Diseases associated with H2AX include Nijmegen Breakage Syndrome and Large B-Cell Lymphoma. Among its related pathways are RNA Polymerase I Promoter Opening and Packaging Of Telomere Ends. An important paralog of this gene is H2AC16.

Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection.