2025年3月10日 · Performance charts for BlackRock Global Funds - Nutrition Fund (H2ZX) including intraday, historical and comparison charts, technical analysis and trend lines.

γH2AX是DNA 损伤尤其是DNA 双链断裂(DNA double-strand breaks,DSB) 或DNA修复的标志物,已经被应用到医学相关领域,如放射性DNA 损伤的检测、癌症的辅助诊断以及预后监测。 此外,γH2AX在检测生殖细胞中的DNA 损伤及修复和维持胚胎干细胞的自我更新中有重要意义。 检测γH2AX水平已成为评价生殖细胞和干细胞质量的重要方法。 本文将从H2AX 及其家族的生物学特征、γH2AX 的检测方法、DNA损伤与修复中的H2AX 磷酸化及其在生殖相关细胞中的应用等角 …

gamma H2A.X为磷酸化蛋白，建议在裂解液中加入复合蛋白酶抑制剂和复合磷酸酶抑制剂，以防止蛋白在提取时降解和去磷酸化。 gamma H2A.X 蛋白预测分子量为15 kDa，建议根据小分子量蛋白实验操作进行实验，详情见关键控制点。 使用依托泊苷诱导处理的HepG2全细胞裂解液. 图三：WB- Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299)。 添加复合蛋白酶抑制剂以避免靶标蛋白降解。 添加复合磷酸酶抑制剂，以防止蛋白在提取时被去磷酸化。 整个 …

2010年2月4日 · Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA double-strand breaks. Detection of this phosphorylation...

Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1). H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1).

Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. γ-H2AX can be detected by immunoblotting and immunostaining using microscopic or flow cytometric detection.

2024年5月24日 · Histone H2AX plays a key role in DNA damage signalling in the surrounding regions of DNA double-strand breaks (DSBs). In response to DNA damage, H2AX becomes phosphorylated on serine residue 139...

2024年2月24日 · Histone γH2AX has a critical role in the cellular DNA repair pathway cascade and contributes to genomic stability. However, the role of γH2AX in the ontology of cancer is unclear. We have...

DNA double-strand breaks are known to be the most lethal kind of DNA damage as they cause genomic instability. Visualization and quantification of these breaks are possible via staining of the phosphorylated histone H2A variant H2AX at serine 139 with the anti-phospho-histone H2AX (γ-H2AX) antibody.

H2AX is a histone H2A variant that constitutes 2–25% of mammalian histone H2A depending on the organism and cell type (Redon et al. 2002; Rogakou et al. 1998).